School of Medicine

Wayne State University School of Medicine

Genotyping

General Information

The Applied Genomics Technology Center is equipped to handle the following genotyping projects:

Sample Requirements

All the sample requirements listed below are guidelines established by the various vendors.  The AGTC staff will work with investigators to optimize these assays for their specific sample types. 

SNP interrogation using TaqMan® 5’ Nuclease Chemistry

  • Submit DNA at a concentration of  5-20 ng/ul
  • For optimal clustering, an effort should be made to normalize all samples to the same approximate concentration
  • Volumes will vary based on project size

SNP interrogation using Illumina GoldenGate® Assay Technology

  • Submit 10ul of DNA at a preferred concentration of 75 ng/ul, although a range of 50-150 ng/ul is acceptable.
  • For optimal clustering, an effort should be made to normalize all samples to the same approximate concentration 
  • Samples are run in 96-well formats.  For custom oligo pools (OPA), synthesis of oligos for a minimum of 480 samples is required.

SNP interrogation using Illumina Infinium® Assay Technology

  • Submit 20ul of DNA at a concentration of 50 ng/ul.
  • The sample concentration range for this assay must be kept very tight for optimal clustering and copy number analysis, therefore the concentration listed above is necessary. 

DNA methylation profiling

  • Submit 25ul of DNA at a concentration of 50ng/ul.
  • The bisulfite conversion process requires 1ug of DNA in a specific volume, therefore the concentration listed above is necessary.

Additional Information

If needed, please contact Matthew Hess for further information reguarding our genotyping services.